1 The assistant uses the right hand to gently press the hen between the pubic bone and the keel, and then uses the right thumb and forefinger to press from the sides to the cloaca and the upper left is the vaginal opening. 2 Inseminators use their right hand to insert an infusion set containing semen into the fallopian tubes 2 to 3 cm deep. When pressing the variator piston, the assistant should relax the hen's abdomen and slowly let the cloaca recover. Do not let the semen bounce out. The sperm delivery device usually uses a 1 ml glass syringe or an ordinary medical pipette. 3 Insemination once a week and hens were given 0.1 ml of semen to obtain more than 95% fertility. If the amount of semen is not enough, it can be diluted 1 to 2 times with normal saline, and it is generally best to use the original sperm. Insemination can be done 4 hours before laying or 1 hour after laying. In the actual work, insemination occurs in the evening to reduce frightening chickens. Eggs collected 2 days after insemination can be used for hatching. The number of fertilized eggs per hen should be counted in time in order to improve artificial sperm retrieval and insemination techniques.