ELX50 washing machine instruction manual

Overview:

BioTek has extensive experience in developing plate washers and automation systems for enzyme-linked immunoassay (EIA) applications, and the ELx50 automatic plate washer is the epitome of years of experience. As a fully functional programmable instrument, the ELx50 provides complete control over the precise transfer of liquids from gentle drip to full pressure delivery systems.

The latest ELx50 washers offer customers the most flexible wash function in the same type of products. Recently, the new ELx50 module can also be used for biomagnetic bead cleaning and filter plate cleaning, allowing users to automate 96-well magnetic beads or poly Cleaning of ethylene microspheres, for example, can complete Luminex's analysis experiments based on the xMAP® technology platform. As the industry leader in orifice cleaning, BioTek incorporates multiple functions in a compact system without the need for additional accessories. The easy-to-use onboard software is easy and flexible to operate. Customers can choose between planar and toroidal magnetic fields to quickly separate micron and nanoscale magnetic beads with high memory. The fast and effective vacuum suction filter module can flexibly adjust the vacuum suction force according to the viscosity of the liquid, and can select different suction filter apertures according to the size of the microspheres. The vacuum filtration module is also suitable for, for example, the removal of residual waste liquid from a purified DNA sample prior to the PCR reaction. The cleaning platform of the washer is also suitable for traditional ELISA cleaning and cell-based cleaning operations of conventional standard solid boards.

The ELx50 is also equipped with a patented Dual-Action manifold in a 16-pass cleaning mode that provides two sets of dispensing and suction lines per hole for unparalleled wash performance. This breakthrough design allows for independent control of the dispensing and suction tubes, providing good full and overfill protection for 96-well and 384-well plates. And can easily adapt to the 384-hole micro-plate area to complete the cleaning work. For 96-well plates, there is no single double pumping method that provides double dispensing and pumping efficiency, ensuring thorough cleaning and residue retention.

The ELx50 washer is widely accepted by experimenters for replacing traditional manual washing, while the new ELx50 is more flexible for multiple washing tasks and fully automated cleaning processes.

Features:

conventional

Syringe's liquid distribution system provides precise control of various liquid flow rates.
Full-board or strip cleaning supports 96- or 384-well microplates. The removable microplate transmitter has a built-in dedicated pre-spray that makes pre-spraying and maintenance simple and convenient for automatic buffer switching (optional)
The onboard software can create, edit, copy, delete, etc. Up to 75 applications can be linked to up to 10 different programs, making it easy to complete complex workflows to support multiple users; not only for comprehensive research applications Programmable control, and provides easy-to-use routine clinical analysis Programmable shock time and intensity Built-in maintenance program Easy to use and maintain Bottom flushing and cross-suction procedures Compact size Built-in dispensing and waste suction Safety dust cover Design creature Magnetic bead separation High-strength magnetic bead separation for magnetic bead-based analysis, such as Luminex's xMAP® analysis
High magnetic 铷 ferromagnetic plate hole inner zone optimized optional planar or toroidal magnetic field - two high-strength 96-well magnetic plates designed for BioTek washer users to quickly separate micro- and nano-microspheres for superior microsphere memory Biomagnetic beads separation Fast and efficient vacuum filtration for polyethylene pellet analysis or waste filtration filtration range from 0.45 mm to 1.2 μmm
Depending on the viscosity of the sample, the optional vacuum level is from 91mmHg to 313mmHg.
Plate vacuum gravity drainage droplets, minimize liquid residue in the plate, avoid cross-contamination
Software control of liquid volume, soaking time, fully automated operation

The main parameters:

Conventional analysis of magnetic beads, polyethylene pellets (depending on model)

Multiple analysis
Microsphere-based ELISA
ELISA

Cell-based analysis

Protein chip

Waste liquid suction filtration (depending on model)

Separation of biomagnetic separation, vacuum filtration (depending on model)
Microplate type 96 and 384-well plates (depending on model)

Standard height and downhole plate

Solid board and filter base plate (depending on model)

Filter hole size: 0.45 μM - 1.2 μM

Liquid transfer built-in positive pressure replaceable syringe pump drive shock preset program can set minutes, seconds to 60 minutes intensity - low, medium, fast or variable soak time preset program can set minutes, seconds to 60 minutes magnetic high intensity 96-well plate format

Available in two designs, BioTek washers are available for users:

flat
Flat bottom - the microsphere is attracted to the bottom of the tape
Round bottom - the microspheres are attracted to the center of the circle - the microspheres are attracted to the periphery of the orifice plate and integrated into the plate frame. The detachable safety/convenience/maintenance waste liquid sensing during non-magnetic cleaning, flow rate detection (optional) )

Dust cover

Overflow protection

Preset maintenance program
Onboard software 2 x 24 digital LCD display

25 Arabic numeric soft keys

Create, edit, or run multiple programs
Operating temperature 15°C to 40°C (59° to 104° F)
Humidity 10% to 85%, non-condensable collector compatible 100 to 240 V~ ± 10% @ 50 - 60 Hz
Cleaning the manifold type 96-/384-well plate cleaning:
Dual-Action™ 16-Channel (1x16) shampoo

96-well plate cleaning:
8-channel (1x8) shampoo
12-channel (1x12) shampoo
Cleaning speed solid board:

Final suction <130 seconds: 3 aspiration/separation cycle, 300 μL/well, 8-channel dispensing head

Filter plate:
Variable, based on cleaning parameters
Vacuum suction optional vacuum level (last 30 seconds):

0.45 μm:
Low: -91 mmHg
Medium: -150 mmHg
High: -313 mmHg

1.2 μm:
Low - : -95 mmHg
Medium - : -155 mmHg
High - : -299 mmHg

Vacuum filtration time range: 5 - 999 seconds Capacity range 25 - 3,000 μL / hole (depending on model)
1 μL stepping lotion selection valve mode supports up to 3 buffers (depending on model)
Flow rate 1 - 9
Cleaning cycle 1 - 10
Liquid separation accuracy ≤ 3% CV

Residual solid bottom plate ≤ 2 μL/well filter plate The average weight per plate is ≤ 1.2 g, and the plate is divided into 300 μL to 0.45 μm, suction filtration for 30 seconds, low vacuum force, blockage
Sterilization Chemistry 70% ethanol or isopropanol and 0.5% sodium hypochlorite supply bottle capacity 2 L
Physical characteristics 16" long x 14" wide x 6.5" high (40.6 x 35.6 x 16.5 cm)
Weight 22 pounds (9.8kg)
Regulations and regulations All BioTek microplate related products are CE and ETL certified.

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